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doi: 10.1146/annurev.immunol.16.1.359 142 PoggiAZocchiMR

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Figure 5 4 Discussion The widespread application of semen cryopreservation technology has effectively prolonged sperm lifespan, overcoming temporal and geographical constraints while maximizing the preservation of superior genetic resources from elite breeding males

SDS-gel electrophoresis and Western blotting SDS-gel electrophoresis and Western blotting were performed as previously described (Stein et al., 2021)