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Subsequently, the percentage recovery of the respective drug samples was calculated

The slides were washed in water and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer

This is particularly relevant because hypoxic tumours are known to exhibit increased resistance to radiotherapy and certain systemic therapies (190, 191), and therefore, imaging hypoxia has potential implications for treatment planning and prognostic assessment

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Zhou and Danbolt, 2014)