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Samples were thawed and lysed using the TissueLyser II (QIAGEN, Hilden, Germany) at a frequency of 30 Hz for 30 s, then submerged in liquid nitrogen to re-freeze, and thawed for 2 min in a water bath

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Furthermore, increased copper levels have been shown to reduce the activity of antioxidant enzymes, such as Cu/Zn-SOD, catalase, and glutathione peroxidase, in the red blood cells, whole blood, live tissue, and brain tissue of rats (67, 68, 69)

Because of this, it would be more beneficial to compare the characteristics of the GF "dough" and bread that was produced with buckwheat and sorghum, those of a real gluten-containing system that is comparable [30, 38]

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