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The following day, cells were washed once with each buffer: low salt (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.0, 150 mM NaCl), high salt (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.0, 5,000 mM NaCl), LiCl (0.25 M LiCl, 1% NP-40, 1% sodium deoxycholate, 1 mM EDTA, 10 mM Tris-HCl pH 8.0) and eluted in 1% SDS, 100 mM NaHCO 3 buffer

Hi, I have just had my first loading dose of B12, and started my period yesterday, normally I hardly notice these but this time I have painful cramps and am a lot heavier than usual

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