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doi: 10.1016/j.lfs.2023.121685 65 SinghN.ThangarajuM.PrasadP

To avoid interference, double blank (blank matrix only) and QC (blank matrix spiked with internal standard only) were also prepared and analyzed

After intervention, the original medium was discarded, and 1 mL of 10 mol/L DCFH-DA working solution prepared in serum-free DMEM was added to each well to ensure complete coverage of the cells

The rats in the fed group had unlimited access to food, whereas those in the fasted group were deprived of food for 18 h prior to the start of the experiment

Zhang Y, Zhang S, Yang L, Zhang Y, Cheng Y, Jia P, et al

10.1111/cen.13830 23 Diamanti-KandarakisE.KouliC.TsianateliT.BergieleA