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The observed increased expression of several proteins involved in carbohydrate catabolism (total 16), including the TCA cycle adds support to the idea of increased energy requirements during nicotine exposure and detoxification

Biofilms were visualised by Confocal Laser Scanning Microscopy (CSLM) (Olympus FV1200, Australia) with Ex/Em 473/559 nm and Ex/Em 500/637 nm for Syto 9 (green for live cells) and PI (red for damaged and dead cells) staining, respectively