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The bacterial cells were harvested, resuspended in binding buffer (20 mM sodium phosphate, pH 7.4, containing 500 mM NaCl and 20 mM imidazole), and lysed by treatment as above, followed by sonication

Since glycolysis is required for acquisition of effector functions ( Figures 2FH )

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Such analyses provides fundamental insight into the biochemical and biophysical determinants of cytoplasmic transport of organelles