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In Caco-2 intestinal epithelial cells, butyrate shows high responsiveness by activating the Nrf2 pathway

The red arrows in Figure 1 show what we call long-range interactions, in which substrates allosterically affect distant enzymes in the network

Binding was visualized by addition of TMB substrate (Calbiochem) or AP-substrate (Merck) (10 g/mL in diethanolamine buffer)

Hydrochloric acid and/or sodium hydroxide may have been added during manufacture to adjust the pH (range 4.5-7.0)

The non-oxPPP in turn adapts to consume or produce pentose phosphates, as controlled by metabolite levels, which determine reaction thermodynamics and thus flux direction

Healthcare professionals may use it in regulated clinical or institutional settings according to local prescribing rules