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Hematoxylin and eosin (H&E) staining was carried out to measure the changes in epidermal thickness

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Kishk A, Pacheco MP, Sauter T

The clean reads were assembled into expressed sequence tag clusters (contigs) and de novo assembled into transcripts by using Trinityin paired-end method ( Functional annotation and differential expression analysis In order to obtain the annotation information of unigenes, the assembled unigenes were annotated with the NCBI non-redundant protein database (NR), Swiss-Prot protein and Clusters of orthologous groups for eukaryotic complete genomes (KOG) databases using BlastX with a threshold E-value of 1.0 10 5