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After removal of the hexanes phase, the pH of the aqueous phase was adjusted to 9.0 with concentrated NH 4 OH solution

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Specifically, adding 5-amino-1mq (30 M and above) to C2C12 myoblast cultures (a common in vitro myoblast model) markedly upregulates the expression of myogenic marker genes, including myosin heavy chain (MyHC), actin, and myogenin (MyoG)