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Once the GST-tagged protein is bound to the glutathione resin, non-specifically bound proteins and contaminants can be washed away using physiologic buffers, such as Tris-buffered saline (TBS) at pH 7.5

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Pharmacologic inhibition of LRRK2 kinase activity rescues these phenotypes, indicating that the effects of G2019S on lysosomal degradative capacity are kinase-specific [182, 183]