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After centrifugation of the reaction, the supernatant solution was collected purified using semi-preparative HPLC, C4 column and gradient of 2060% B to give the desired product in ~50% yield, (Supplementary Fig

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It is important to be gentle during this process to avoid damaging the delicate material

405 A prospective, randomized, blinded study compared the effects of topiroxostat with allopurinol and suggested that topiroxostat may lead to a reduction in left ventricular end-diastolic pressure, indicating a potential benefit for cardiac function