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To further prove the influence of intracellular GSH level on the cytotoxicity of NSBSO, we pre-treated 4T1 and B16 cells with GSH synthesis inhibitor to reduce the intracellular GSH level, and the results showed that the pretreatment of the inhibitors gradually reduced the intracellular GSH to a very low level after 24 h treatment (Additional file 1: Fig

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[DOI] [PubMed] [Google Scholar] 58.Smith A.E., Walter A.A., Graef J.L., Kendall K.L., Moon J.R., Lockwood C.M., Fukuda D.H., Beck T.W., Cramer J.T., Stout J.R

weight loss started from week 4, progressed steadily, and reached a nadir at week 60, showing progressive reduction over time, though specific intermediate monthly averages (e.g., at 3, 6, or 12 months) are not detailed in the main trial publication

111 CatalanDMansillaMAFerrierASotoLOleinikaKAguillonJCet al

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