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34798964 Fan JM, Singhal NS, Guterman EL

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3T3-L1 cells were cultured in 60 mm diameter dishes (8.4104 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)

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The State has rules and regulations for using the machine and making sure its working correctly