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Glutathione peroxidase (GPx) was tested in tissue supernatant using assay buffer (phosphate buffer 0.15 M and EDTA, 5 mM) with NADPH, glutathione reductase (GR), glutathione (GSH), and hydrogen peroxide as substrate for glutathione oxidation

The l-EV fraction from cells expressing the MIM 5KA mutant with Cas12f failed to induce GFP expression in recipient reporter cells (Fig