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Harvested cells were lysed, fractionated and conjugated to a rhodamine-azide tag using copper-catalyzed azide-alkyne cycloaddition (CuAAC) chemistry 25 , enabling visualization of probeprotein interactions via gel-based fluorescence scanning

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The maximum laccase activity detected at 32.7 C was defined as 100% (initial activity) and other temperature points were normalized to that activity (relative activity)

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Forman, S