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They exhibit low ion-leaching properties even in chemical additives such as chelators (EDTA), strong reducing agents (DTT), or components of cell culture supernatants, which typically strip off Ni ions and reduce the functionality of most IMAC magnetic beads

doi: 10.1038/s41419-019-1984-4 73 WuJMinikesAMGaoMBianHLiYStockwellBRet al

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The cell-stress response to this event, known as the unfolded protein response (UPR) activates cascade of reactions and pathways leading to cell death by apoptosis (Xu et al., 2005)