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The cut-off between normal vitamin B12 levels and deficiency varies by method and laboratory, but most laboratories define subnormal serum or plasma values as those lower than 200 or 250 pg/mL (148 or 185 pmol/L)

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The B12-intrinsic factor complex travels to the terminal ileum, where it binds to specific receptors (cubilin-amnionless complex) and is absorbed through receptor-mediated endocytosis