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miRNAs were shown to regulate metabolism-related genes in T1D, an autoimmune disease characterized by persistent hyperglycemia secondarily to pancreatic -cell destruction and insulin deficiency (118, 119)

Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting

- Dynabeads magnetic beads for positive isolation for molecular downstream assays: Positive isolation of target cells without bead release can be used when the aim is downstream molecular studies such as DNA, RNA, or protein analysis

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However, further in vitro assays and preclinical studies with this NP-delivered metal chelator approach are warranted to improve their targeting capacity to the brain as well as to estimate the level of BBB crossing, chelation efficiency and the related toxicity before clinical applications