Protein pellet was resuspended in 1 mL of 6 M guanidine hydrochloride at 37C for 30 min with vortex mixing
Accumulating in vitro evidence indicates that nuclear HDAC13 are the most potent lysine delactylases, whereas SIRT13 also exhibit substantial delactylation activity toward both L- and D-lactyl modifications
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Li F, Singh AV (2021) Recent advancements to enhance the therapeutic efficacy of antiepileptic drugs
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Schematically: Enzyme-His-phosphate + 3-PG Enzyme-His + 2,3-BPG Enzyme-His + 2,3-BPG Enzyme-His-phosphate + 2-PG It is important to note that the phosphoryl group in 2-phosphoglycerate is not the same as the one originally present in 3-phosphoglycerate
