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Animal studies demonstrate enhanced collagen organization and improved biomechanical strength in healing tendons treated with the peptide

After the treatment with test samples for 48 h, the medium was replaced with fresh medium every three days and cultured for 1014 days

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66 Subsequently, downstream transcription factors including nuclear factor-B (NF-B), activator protein-1 (AP-1), and IFN regulatory factor 3/7 (IRF3/7) are activated to promote the transcription of pro-inflammatory cytokines and IFN-I

Furthermore, to verify the reliability of these targets, molecular docking was performed using AutoDockTools 1.5 and PyMOL, in combination with the PDB database ( Finally, Rg1 presented 100 potential targets, which matched with a set of 9,366 PD-related targets to produce 72 shared targets for examination