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Fauvet et al., 2012

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Array-based DNA-methylation profiling in sarcomas with small blue round cell histology provides valuable diagnostic information

Biochemical analyses Transfected N2a cells were lysed with ice-cold Lysis buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM MgCl 2 , 2 mM EDTA, 1% Triton X-100, 0.1% SDS, and 10% glycerol) supplemented with a protease inhibitor cocktail (Roche)

Tet oxidizes thymine to 5-hydroxymethyluracil in mouse embryonic stem cell DNA