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doi:10.1152/ajpregu.1992.263.5.R992 Lee, Eunjoo et al

It is recommended to give the Botox a minimum of 2 rounds 3 months apart to get a good sense of how much benefit one can likely expect

cDNA constructs of GST fusion proteins were incorporated in pGEX-6p-2 plasmid (GE Healthcare Life Sciences, Little Chalfont, USA) and transformed a bacterial line BL21