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There is no single correct amount more water simply spreads the same 1000 mg of peptide across a larger volume, which makes small doses easier to measure accurately

FIGURE S3 Quantification of live/dead biofilm biomass of MRAB-3

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Under oxidative stress, excessive ROS such as OH directly attack DNA, causing single-strand or double-strand breaks, activating PARP-1, consuming NAD and ATP, and generating poly(ADP-ribose), resulting in mitochondrial membrane potential loss and the release of apoptosis-inducing factor, ultimately resulting in cell death [252]

Never shake vigorously, as this can cause peptide aggregation and loss of activity