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HLA-I peptides were separated with a 60-min gradient from 3% to 25% acetonitrile in 5% DMSO/0.1% formic acid, while HLA-II peptides used a gradient from 3% to 30% acetonitrile, both at 250nl/min flow rate

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Serum from RBD-NP-immunized nonhuman primates strongly cross-reacted with Pangolin-GD and RaTG13 RBDs but had weaker binding to distantly related RmYN02, SARS-CoV-1, WIV16, and ZXC21 RBDs [146]