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For NADP + and NADPH assays, 20 L (for NADP + assay) or 30 L (for NADPH assay) of the neutralized sample extract was combined with 90 L of assay mix consisting of 50 L of 0.1 M HEPES containing 2 mM EDTA (pH 7.5), 20 L of 1.2 mM 2,6-dichlorophenolindophenol, 10 L of 20 mM phenazine methosulfate, and 10 L of 10 mM glucose-6-phosphate

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It included subjects with NAFLD, who were or were not obese, and evaluated if, and how, their plasma amino acids were altered, and the relationship with insulin resistance

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After developing an analytical method to quantify polyphenol concentrations in saliva, a saliva sample and a simultaneously obtained serum sample of a single volunteer were analyzed after intake of Pycnogenol (70)