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Pyroptosis depends on inflammasomes, caspase-1/11, gasdermin-D (GSDMD), and the release of interleukin-1 (IL-1 ) and IL-18 (30)

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After 30 minutes at 37C, 500 L of cold ethanol was added to the homogenate to precipitate proteins, followed by centrifugation (21,000 g , 4C, 10 minutes), and the supernatant was dried under an N 2 stream at room temperature

The resulting O 2 oxidatively activates the epithelial MAPK/AP-1 pathway, promoting the release of IL-33 and TSLP

doi: 10.1016/j.biopha.2024.116703 284 ZhaoL.XuH.LiuX.ChengY.XieJ

Zhou SY, Gillilland M 3rd, Wu X, Leelasinjaroen P, Zhang G, Zhou H, Ye B, Lu Y, Owyang C