Saline, Zofran, Toradol, and Pepcid
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GF: Data curation, Investigation, Methodology, Resources, Writing original draft
Antibodies The following antibodies were used for western blotting (WB) and/or immunofluorescence staining (IF) at the following dilutions: mouse anti-CIP4 (BD, clone 21, 612556, 1:100 for IF, 1:1000 for WB), mouse anti-GAPDH (Santa Cruz, sc-166574, 1:2000 for WB), rabbit anti-WAVE2 (Cell signaling, clone D2C8, #3659, 1:100 for IF, 1:1000 for WB), rabbit anti-N-WASP (Cell signaling, clone 30D10, #4848, 1:1000 for WB), mouse anti-dynamin II (BD, #610263, 1:1000 for WB), mouse anti-endophilin II (Santa Cruz, #sc-365704, 1:1000 for WB), rabbit anti-clathrin light chain A (CLTA) (proteintech, #10852-1-AP, 1:1000 for WB), mouse anti-Rac1 (Millipore, 05-389, 1:4000 for WB), mouse anti-Rac1 (BD, #610651, 1:200 for WB of EV uptake, 1:400 for ELISA), rabbit anti-Cdc42 (Cell signaling, #2462, 1:1000 for WB), rabbit anti-HaloTag (Promega, #G9281, 1:1000 for WB, 1:1000 for IF, 1:1000 for ELISA), mouse anti-HaloTag (Promega, #G9211, 1:1000 for WB, 1:1000 for ELISA), mouse monoclonal anti-MIM (Abnova, #H00009788-M01, 1:1000 for WB), mouse anti-CD81 (Santa Cruz, #sc-166029, 1:2000 for WB), mouse anti-Annexin I (Santa Cruz, sc-12740, 1:1000 for WB), rabbit anti-EEA1 (Cell signaling, #3288, 1:100 for IF), mouse anti-Lamp1 (Santa Cruz, #sc-20011,1:50 for IF), mouse anti--actin (Santa Cruz, #sc-47776, 1:3000 for WB), mouse anti-mCherry (Novus, #NBP1-96752, 1:1000 for IF), rabbit anti-GFP (MBL Life Science, #598, 1:4000 for WB), and rabbit anti-RFP (MBL Life Science, #PM005, 1:3000 for WB)
Japanese populations consume significantly more taurine (through seafood and organ meats) than Western populations, which may contribute to their lower cardiovascular disease rates
The route is subcutaneous injection with a small insulin-size needle, usually into the abdomen or thigh, with daily site rotation
