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After administration blood samples were collected at time points 0, 1 , 2, 4, 8, 12, 24 and 48 hours and kept frozen until analysis

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MCF-7 cells were grown in 100 mm* 20 mm cell culture dishes (Corning, USA) until 80-90% confluency

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Whole-cell patch-clamp recordings were performed on mRFP--actin-transfected hippocampal neurons to measure miniature excitatory postsynaptic currents (mEPSCs), reflecting synaptic activity [1]

2 ) decreased neurotransmitter vesicle and mitochondrial anterograde and retrograde transport along microtubules that would lead to decreased neurotransmission and to diminished levels of needed ATP to maintain synaptic membrane ion gradients (-tubulin, neurofilament protein medium)