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Integrated heats were fitted using the Microcal PEAQ-ITC analysis software v1.30 (Malvern Panalytical) to obtain n , K d , H , and T S

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Primary mouse hepatocytes were collected through ultrasonication with phosphate buffer solution in an ice-water bath after the intervention, and the resulting centrifugal supernatants were gathered

Selected E-64 homologous biosynthetic gene clusters in clinker visualization, including reported E-64 analog producing fungi ( Aspergillus oryzae , Penicillium citrinum , and Colletotrichium spp ) and fungi not known to produce E-64 ( Trichoderma atroviride , Metarhizium anisopliae , and Mycena galopus ATCC 62051)