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coli NEBExpress was first transformed with the pKD46 plasmid and then typically grown in 2 L Terrific Broth (TB) medium supplemented with 100 g/mL Amp at 30 C in the presence of 0.2 % (w/v) arabinose to induce expression of the Red genes

the decrease in HGF-dependent MDCK proliferation below control levels for some compounds is not surprising since the experiment was carried in 2% serum, which likely contains some level of HGF

In Situ Metabolic Characterisation of Breast Cancer and its Potential Impact on Therapy