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The resuspended samples were transferred into Lysing Matrix B tubes (MPBio) and vortexed three times for 45 s each, allowing the samples to cool on ice between vortexing periods

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These gains have been documented in numerous studies and are associated with elevated physical activity levels both before and after surgery (74)

SLC39A1 lko mice displayed reduced tumor number in DEN-CCL4 model compared with SLC39A1 flox/flox mice, evidenced by reduced liver damage, hepatocyte proliferation (Ki-67), inflammation (TGF-), and fibrosis (Masson), accompanied by increased ROS accumulation and decreased LC3 and Lamp1