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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

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J Neurosci 23 : 1142711435

This is believed to be due to high blood sugar interfering with and degrading sensitive nerve fibers

Drugs which have been classified as confirmed or potential nootropics are often those initially investigated for the amelioration of cognitive dysfunction in aging and neurodegenerative disorders, as well as the treatment of attention or learning deficits in such conditions as attention deficit hyperactivity disorder (ADHD) or autism spectrum disorders