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Myeloperoxidase (MPO) activity, a marker for neutrophil infiltration, was measured in lung tissue homogenates using a previously described spectrophotometric method, assessing HO-dependent o-dianisidine oxidation

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The authors did observe some inter-metastatic heterogeneity, but these heterogeneous mutations were predicted to be nonfunctional

It is crucial to note, however, that recent studies highlight significant systemic side effects of MCT4 inhibition due to its broad tissue expression, which may limit its clinical translational potential and necessitate the development of tissue-specific delivery strategies [138]

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