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A 31G needle connected to a saline infusion set was carefully inserted into the mouses anterior chamber and the anterior chamber pressure was gradually increased to 120 mmHg by adjusting saline bottle height (162 cm)

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Indications for a direct connection between the nose and the CNS were discovered at the beginning of the last century [8], as researchers were able to show that substances injected into the subarachnoid space can reach the nasal mucosa [21]

Additionally, the coagulation process is regulated by other mechanisms to ensure that thrombi form only when necessary and to prevent excessive thrombus formation