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Those observations are included here only to summarize research context

Generation of gradients on a microfluidic device: toward a high-throughput investigation of spermatozoa chemotaxis

The timing consideration isn't about separation windows

Quality control calibration procedures was performed on a spine phantom (Hologic X-CALIBER Model DPA/QDR-1 anthropometric spine phantom) and a density step calibration phantom prior to each testing session

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431 Meanwhile, ZIP6, another downstream target of STAT3, is involved in cancer development by forming a heterodimer with ZIP10