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For qPCR analysis, 5 L of immunoprecipitated DNA was used as a template in a 10 L total reaction volume using TaqMan Advanced PCR Master Mix (Thermo Fisher Scientific) and target-specific TaqMan probes, prepared in 384-well plates

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10.3390/nu14153042 25 ChenJ.LiX.GeC.MinJ.WangF

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PMID: 40055321 NPJ Biofilms Microbiomes Gut microbiota dysbiosis-mediated ceramides elevation contributes to corticosterone-induced depression by impairing mitochondrial function