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Bound HA specific mouse antibodies were detected using an AP-conjugated anti-mouse IgG Fc specific antibody from goat (Sigma-Aldrich, A9544) (diluted 1:4000 in PBS/T/S) and visualized by addition of AP-substrate (10 g/mL in diethanolamine buffer)

Thats because your heart may beat extra hard or fast to try to move red blood cells through your body