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After 48 h, the measurement was started by switching from DMEM medium to preheated glucose starvation minimal medium (140 mM NaCl, 5 mM KCl, 1 mM of MgCl 2 , 2 mM of CaCl 2 , 20 mM Hepes, pH 7.4 as adjusted with NaOH) with 10% FCS

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Hence, to the best of our knowledge, we optimized acellular kidney scaffolds harvested from human fetal kidneys for the first time by comparing the outcomes following various SDS concentrations, each at either 24 or 48-h duration of perfusion

amin E group, compared with 440 days for the 1990;24:109