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Hang B, Jassem E, Mohammed H, Wan LQ, Herschkowitz JI, Fan J

*Correspondence: Mercedes Gil-Campos [email protected] This article was submitted to Nutrition and Metabolism, a section of the journal Frontiers in Nutrition Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

These methodologies are demonstrated on an antibody fragment, and serve to expand the scope of disulfide bridging strategies whilst offering a convenient route to the construction of multifunctional antibody fragment conjugates

Stomach Pumping : Also known as gastric lavage, this procedure removes unabsorbed alcohol from the stomach if performed within an hour of ingestion

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The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)