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Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 10 6 cells/mL for 2 h in serum-free media at 37 C [20]

Finally, VEGF secreted in the co-culture system may get bound to the extracellular matrix, where it may not be accessible to VEGF antibodies, but may still be able to be affected by receptor inhibition

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Accumulating evidence has demonstrated that BCAT1, functioning as a key promoter of tumor metastasis and proliferation, holds paramount significance not only in the early detection of tumors but also plays a pivotal role at the treatment phase [45]

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