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Analysis of calcium ion (Ca 2+ ) levels in macrophages To detect the intracellular Ca 2+ content, 143B cells were incubated with PBS, Ru(II)-OH, NP2, and NP4 (Ru 10 M) for 6 h

To test the functional involvement of M2 macrophages in fibrotic pathology, we first induced macrophage polarization in vitro by using LPS or IL-4 into either M1 or M2 phenotypes, respectively (Additional file 1: Figure S3)