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identified 17 epitopes from shrimp TM and validated them as capable of inducing T cell proliferation and cytokine release (IL-6 and IL-13) in shrimp-allergic individuals

The concentration of trichloroacetic acid should not exceed this value to avoid complicating subsequent staining of samples in the reaction with Ellmans reagent [9]

Luminescence measurement was performed by multi-mode microplate reader (FLUOstar Omega)

Note: cooking collagen turns it to gelatine, which provides the body with amino acids, the building blocks of proteins

doi: 10.1007/s10787-024-01499-8

10.1254/jphs.fp0072161 65 KolovratM.GojkovicS.KrezicI.MalekinusicD.VrdoljakB.Kasnik KovacK.et al (2020)