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Description

Mobile phase A and B was 0.2% (v/v) AA in water (Optima grade, Fisher Scientific, USA) and 0.2% (v/v) AA in LC-MS grade acetonitrile (Honeywell, USA), respectively

coli cells (F- mcrA ( mrr-hsd RMS- mcr BC) 80 lac ZM15 lac X74 rec A1 ara D139 gal U gal K ( ara-leu )7697 rps L (Str R ) end A1 nup G) were used for general plasmid DNA propagation and A