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Air-dried smears were fixed in Carnoys solution (methanol: acetic acid, 3:1) for 2 h, stained with AO solution (0.19 mg/mL AO in phosphate-citrate buffer, pH 2.5) for 5 min, and immediately examined under a fluorescence microscope

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[DOI] [PMC free article] [PubMed] [Google Scholar] 62.Loebel M, Grabowski P, Heidecke H, Bauer S, Hanitsch LG, Wittke K, et al