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Glutathione levels are maintained in cells through de novo production, as well as through the recycling of its oxidized form, glutathione disulfide (GSSG)

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Importantly, endogenous GSH production begins to decline after age thirty, increasing vulnerability to environmental insults and chronic disease

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To determine whether these transcriptomic changes were reflected at the metabolic level, we quantified -hydroxybutyrate, a terminal product of -oxidation, in both cell lysates and culture supernatants