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Reference genes, RPL32 and GUSB , were used to normalize expression of mRNAs of interest

Figure 1 illustrates the 3R strategy framework

In one study, GHK-Cu was shown to increase hair follicle size and stimulate the proliferation of dermal papilla cellscritical for initiating new hair growth (Pickart & Margolina, 2018)

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Alternatively, oligonucleotides that bind double stranded DNA to form a ternary construct to prevent DNA unwinding and transcription can be used