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Reversibility and thiol dependence of APR-246 binding to Trx1 and Grx1 Reduced Trx1 and Grx1 were incubated with 240 and 1000 M APR-246, respectively for one hour at 37 C prior to assessment of protein thiol content with DTNB as described above

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Better animal models are needed in order to recapitulate human disease, diverse antigenic history (mixed infection and vaccination) and immune phenomena such as original antigenic sin and imprinting