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Serum levels of the potent inflammatory cytokine, IL-1, are elevated in IBD patients (Ye et al., 2020)

Electrolytes Electrolytes include sodium, potassium, magnesium, phosphorous and calcium

Invest 123 (3), 951957

After 72 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution (25 L/well, at a final concentration of 5 mg/mL) was added to each well 4 h before the end of the culture

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it's a professional protocol born from years of lab work, designed to ensure the integrity of your research from the very first step