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By using Cytoscape software (Version: 3.10.2) in conjunction with MCC algorithms of the CytoHubba plugin, we identified the top 5 hub genes within the network (IL1B, NFKB1, MAP2K1, PRKACA, and HSP90AA1), suggesting that these genes are potential targets for EMPA and MET in heart failure therapy (Figure 6C)

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The isolated cells were cultured at 37 C in a humidified 5% CO 2 incubator for 2 h